Boster provides high-quality ELISA kits that are cited in over 6,000 publications
Boster Biological Technology has over 25 years of experience manufacturing ELISA kits and antibodies. As a trusted ELISA kit company, we are proud to offer over 1,000 ELISA test kits in both sandwiches ELISA and competitive ELISA formats, covering humans, mice, rats, and other mammalian species. As life scientists, we understand the competitive and challenging nature of your work, and we go to great lengths to support your experiments, from design to data analysis.
Boster Enzyme-Linked Immunosorbent Assays undergo meticulous and robust validation to ensure the highest specificity, sensitivity, and stability. Use Boster’s ELISA test kits and you’ll get reproducible and publishable data batch after batch. CiteAb.com, a big data research company, rated us as the fastest-growing antibody company in the world in terms of publication citations. As of 2019, our ELISA kits alone have been cited in over 6,000 publications worldwide.
How does the ELISA kit work?
The key step, immobilization of the antigen of interest, can be achieved by direct adsorption to the assay plate or indirectly through a capture antibody that has adhered to the plate. The antigen is then detected directly (enzyme-labelled primary antibody) or indirectly (enzyme-labelled secondary antibody). Detection antibodies are usually labelled with alkaline phosphatase (AP) or horseradish peroxidase (HRP). A large selection of substrates is available to perform ELISA with an HRP or AP conjugate.
The choice of substrate depends on the assay sensitivity required and the instrumentation available for signal detection (spectrophotometer, fluorometer, or luminometer). Detection is achieved by measuring enzyme activity. One method is through incubation with TMB substrate which can be detected through the light absorbance of the converted substrate at 450nm. Alternatively, the enzyme can catalyze a chemiluminescent substrate, triggering a strong luminescent reaction that can be quantified.